Document Type

Article

Publication Title

RNA: A Publication of the RNA Society

Publication Date

7-1999

Abstract

Positioning rRNA within the ribosome remains a challenging problem. Such positioning is critical to understanding ribosome function, as various rRNA regions interact to form suitable binding sites for ligands, such as tRNA and mRNA. We have used phenanthroline, a chemical nuclease, as a proximity probe, to help elucidate the regions of rRNA that are near neighbors of the stem-loop structure centering at nt 790 in the 16S rRNA of the Escherichia coli 30S ribosomal subunit. Using phenanthroline covalently attached to a DNA oligomer complementary to nt 787-795, we found that nt 582-584, 693-694, 787-790, and 795-797 were cleaved robustly and must lie within about 15 Angstrom of the tethered site at the 5' end of the DNA oligomer, which is adjacent to nt 795 of 168 rRNA.

DOI

10.1017/S1355838299990143

Comments

© 1999, authors. View original published article at RNA: A Publication of the RNA Society.

Rights

© 1999, authors. View original published article at RNA: A Publication of the RNA Society.

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

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Biology Commons

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