Presentation Title

Dynamic, Reversible 3D Cell Culture Matrices

Presentation Type

Oral Presentation

Abstract

Three-dimensional (3D) cell cultures are becoming the research tool of the future as they closely mimic the physiological environment of cells. A major drawback of current 3D culturing systems is the inability to easily recover entrapped cells for subsequent analyses or subculturing. In this context, we developed a dynamic, reversible 3D cell culture system based on hyaluronic acid and gelatin. Our data so far indicate that the hydrogels are thixotropic, are stable at physiological temperatures, do not display significant amounts of swelling in aqueous environments, and are cytocompatible. Furthermore, these matrices allow for facile recovery of entrapped cells by simple centrifugation. Overall, our results indicate that these materials could potentially revolutionize the traditional cell culturing methods by enabling efficient, large scale, high throughput cell cultures in 3D.

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Apr 27th, 2:05 PM Apr 27th, 2:20 PM

Dynamic, Reversible 3D Cell Culture Matrices

UC Ballroom, Pod #4

Three-dimensional (3D) cell cultures are becoming the research tool of the future as they closely mimic the physiological environment of cells. A major drawback of current 3D culturing systems is the inability to easily recover entrapped cells for subsequent analyses or subculturing. In this context, we developed a dynamic, reversible 3D cell culture system based on hyaluronic acid and gelatin. Our data so far indicate that the hydrogels are thixotropic, are stable at physiological temperatures, do not display significant amounts of swelling in aqueous environments, and are cytocompatible. Furthermore, these matrices allow for facile recovery of entrapped cells by simple centrifugation. Overall, our results indicate that these materials could potentially revolutionize the traditional cell culturing methods by enabling efficient, large scale, high throughput cell cultures in 3D.