Disruption of the Borrelia Burgdorferi Gac Gene, Encoding the Naturally Synthesized GyrA C-Terminal Domain

Authors

Scott W. KnightFollow
Betsy J. KimmelFollow
Christian H. EggersFollow
D. Scott Samuels, University of Montana - MissoulaFollow

Document Type

Article

Publication Title

Journal of Bacteriology

Publication Date

4-2000

Volume

182

Issue

7

Disciplines

Biology | Life Sciences

Abstract

The C-terminal domain of the A subunit of DNA gyrase, which we term Gac, is naturally synthesized in Borrelia burgdorferi as an abundant DNA-binding protein. Full-length GyrA, which includes the C-terminal domain, is also synthesized by the spirochete and functions as a subunit of DNA gyrase, We have disrupted synthesis of Gac as an independent protein and demonstrated that it is not essential for growth in a coumarin-resistant background. We detected no alterations in DNA maintenance, condensation, or topology In B. burgdorferi lacking this small DNA-binding protein.

DOI

10.1128/JB.182.7.2048-2051.2000

Recommended Citation

Knight, Scott W.; Kimmel, Betsy J.; Eggers, Christian H.; and Samuels, D. Scott, "Disruption of the Borrelia Burgdorferi Gac Gene, Encoding the Naturally Synthesized GyrA C-Terminal Domain" (2000). Biological Sciences Faculty Publications. 163.
https://scholarworks.umt.edu/biosci_pubs/163