Journal of Bacteriology
Biology | Life Sciences
The C-terminal domain of the A subunit of DNA gyrase, which we term Gac, is naturally synthesized in Borrelia burgdorferi as an abundant DNA-binding protein. Full-length GyrA, which includes the C-terminal domain, is also synthesized by the spirochete and functions as a subunit of DNA gyrase, We have disrupted synthesis of Gac as an independent protein and demonstrated that it is not essential for growth in a coumarin-resistant background. We detected no alterations in DNA maintenance, condensation, or topology In B. burgdorferi lacking this small DNA-binding protein.
Knight, Scott W.; Kimmel, Betsy J.; Eggers, Christian H.; and Samuels, D. Scott, "Disruption of the Borrelia Burgdorferi Gac Gene, Encoding the Naturally Synthesized GyrA C-Terminal Domain" (2000). Biological Sciences Faculty Publications. 163.