Year of Award
Master of Science (MS)
Department or School/College
Department of Chemistry
Bruce Bowler, Stephen Lodmell
University of Montana
Aptamer science is a growing field of both chemistry and biochemistry. Aptamers bind to specific target molecules, potentially allowing for identification and quantification. The methodologies for selection of aptamers are growing and ever-changing. There is a number of different selection protocols, some specialized and others more general. All have their advantages and limitations. Here I describe a host of these protocols and relate them towards the selection of an aptamer for oxidative DNA lesions, specifically the oxidation products of guanine. Guanine has the lowest reduction potential of the four DNA bases, and as such is the most readily oxidized. The oxidation product 7,8-dihydro- 8-oxoguanine (8-oxoG) has been studied extensively over the last decade, but the further oxidation products Spiroiminohydantoin (Sp), Guanidinodihydantoin (Gh), and Iminoallantoin (la) are still largely unresearched. Aptamers for these products would prove to be invaluable diagnostic tools for the measurement of oxidative damage to DNA. Attempts to select aptamers toward these compounds are described, and recommendations for further attempts at aptamer selection are provided.
James, Brandon Lloyd, "Aptamer Selection for Oxidative DNA Lesions" (2009). Graduate Student Theses, Dissertations, & Professional Papers. 10833.
© Copyright 2009 Brandon Lloyd James