Document Type
Article
Publication Title
Journal of Biological Methods
Publication Date
3-2018
Volume
5
Issue
1
Disciplines
Biology | Life Sciences
Abstract
Genome analysis using pulsed-field gel electrophoresis (PFGE) has been used in applications ranging from typing bacterial strains to radiobiology to cancer research. While methods for running PFGE have been significantly improved since its invention, the method for preparing chromosomal DNA itself has remained essentially unchanged. This limits the applicability of PFGE, especially when analyses require many samples. We have streamlined sample preparation for routine applications of PFGE through the use of deep-well 48-well plates. Besides saving time, our protocol has the added advantage of reducing the volume of expensive reagents. Our improved protocol enables us to reduce throughput time and simplify the procedure, facilitating wider application of PFGE-based analyses in the laboratory.
Keywords
DNA plugs; chromosomal DNA preparation; deep-well 48-well plate; genomic fingerprinting; karyotyping; pulsed-field gel electrophoresis
DOI
10.14440/jbm.2018.218
Rights
Copyright © 2013-2018 The Journal of Biological Methods, All rights reserved.
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
Recommended Citation
Hicks L, van der Graaf CM, Childress J, Cook E, Schmidt K, Rosenzweig F, Kroll E. Streamlined preparation of genomic DNA in agarose plugs for pulsed-field gel electrophoresis. J Biol Methods 2018;5(1):e86. DOI: 10.14440/jbm.2018.218