Document Type
Article
Publication Title
Infection and Immunity
Publisher
American Society for Microbiology
Publication Date
12-1991
Volume
59
Issue
12
Disciplines
Biology | Life Sciences
Abstract
The cbbE' gene codes for the E' protein of Coxiella burnetii and was detected in genomic DNA from all known human isolates of the biotzere strain but not in DNA from the other five strains of C. burnetti. The biotzere strain is strictly associated with chronic disease in humans. Extrinsic iodination of biotzere strain cells radiolabeled a 55-kDa protein which comigrated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with the E' protein synthesized in vitro from recombinants containing the cbbE' gene. The 125I-labeled 55-kDa protein was immunoprecipitated with polyclonal anti-E' antiserum, confirming its identity as E'. Predicted secondary structure of the E' polypeptide shows six regions of beta-sheet structure and an alpha-helix near the C terminus with adequate lengths to span a membrane. The predicted hydropathy profile of E' is similar to profiles of known outer membrane proteins and corroborates the biochemical data, indicating that the protein is located in the outer membrane of C. burnetii.
DOI
https://doi.org/10.1128/iai.59.12.4735-4739.1991
Rights
© 1991 American Society for Microbiology
Recommended Citation
Minnick, Michael F.; Heinzin, Robert A.; Reschke, Dennis K.; Frazier, Marvin Eugene; and Mallavia, Louis P., "A plasmid-encoded surface protein found in chronic-disease isolates of Coxiella burnetti" (1991). Biological Sciences Faculty Publications. 472.
https://scholarworks.umt.edu/biosci_pubs/472