Crystal structure of the adenylyl cyclase activator G(sα)

Authors

Roger K. Sunahara, University of Texas Southwestern Medical Center
John J.G. Tesmer, Howard Hughes Medical Institute
Alfred G. Gilman, University of Texas Southwestern Medical Center
Stephen R. Sprang, University of MontanaFollow

Document Type

Article

Publication Title

Science

Publication Date

12-12-1997

Volume

278

Issue

5345

Disciplines

Biology | Life Sciences

Abstract

The crystal structure of G(sα), the heterotrimeric G protein α subunit that stimulates adenylyl cyclase, was determined at 2.5 Å in a complex with guanosine 5'-O-(3-thio-triphosphate) (GTPγS). G(sα) is the prototypic member of a of GTP-binding proteins that regulate the activities of effectors in a hormone-dependent manner. Comparison of the structure of G(sα)·GTPγS with that of G(iα)·GTPγS suggest that their effector specificity is primarily dictated by the shape of the binding surface formed by the switch II helix and the α3-β5 loop, despite the high sequence homology of these elements. In contrast, sequence divergence explains the inability of regulators of G protein signaling to stimutate the GTPase activity of G(sα). The βγ binding surface ofG(sα) is largely conserved in sequence and structure to that of G(iα), whereas differences in the surface formed by the carboxyl-terminal helix and the α4-β6 loop may mediate ceptor specificity.

DOI

https://doi.org/10.1126/science.278.5345.1943

Recommended Citation

Sunahara, Roger K.; Tesmer, John J.G.; Gilman, Alfred G.; and Sprang, Stephen R., "Crystal structure of the adenylyl cyclase activator G(sα)" (1997). Biological Sciences Faculty Publications. 536.
https://scholarworks.umt.edu/biosci_pubs/536