Crystallization and preliminary crystallographic studies of giα1 and mutants of giα1 in the GTP and GDP-bound states

Authors

David E. Coleman, University of Texas Southwestern Medical Center
Ethan Lee, University of Texas Southwestern Medical Center
Mark B. Mixon, University of Texas Southwestern Medical Center
Maurine E. Linder, University of Texas Southwestern Medical Center
Albert M. Berghuis, University of Texas Southwestern Medical Center
Alfred G. Gilman, University of Texas Southwestern Medical Center
Stephen R. Sprang, University of Montana

Document Type

Article

Publication Title

Journal of Molecular Biology

Publication Date

1994

Volume

238

Issue

4

Disciplines

Biology | Life Sciences

Abstract

Several different crystal forms of Giα1 have been grown and analyzed. Crystals of native protein containing bound GTPγS belong to space group P3₁21 or P3₂21 with cell dimensions a, b = 80·6 Åand c = 106·3 Å and diffract to a resolution of 1·9 Å using synchrotron radiation. Crystals of native protein containing bound GDP belong to space group I4 with cell dimensions a, b = 121·3 Å, and c = 67·7 Å and diffract to 3·0Å. Data sets from crystals grown using mutant proteins have also been obtained and characterized.

DOI

https://doi.org/10.1006/jmbi.1994.1320

Rights

© 1994 Academic Press Ltd.

Recommended Citation

Coleman, David E.; Lee, Ethan; Mixon, Mark B.; Linder, Maurine E.; Berghuis, Albert M.; Gilman, Alfred G.; and Sprang, Stephen R., "Crystallization and preliminary crystallographic studies of giα1 and mutants of giα1 in the GTP and GDP-bound states" (1994). Biological Sciences Faculty Publications. 549.
https://scholarworks.umt.edu/biosci_pubs/549