Document Type
Article
Publication Title
Applied and Environmental Microbiology
Publication Date
2-2010
Volume
76
Issue
3
Disciplines
Biology | Life Sciences
Abstract
We have demonstrated that rpsL, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in Borrelia burgdorferi, the causative agent of Lyme disease. Mutations in rpsL confer streptomycin resistance. Streptomycin susceptibility is dominant in an rpsL merodiploid, and streptomycin selects for the loss of wild-type rpsL carried in trans. This is the first description of a counterselectable marker in B. burgdorferi.
DOI
10.1128/AEM.02172-09
Recommended Citation
Drecktrah, Dan; Douglas, Miles J.; and Samuels, D. Scott, "Use of Rpsl as a Counterselectable Marker in Borrelia Burgdorferi" (2010). Biological Sciences Faculty Publications. 57.
https://scholarworks.umt.edu/biosci_pubs/57