Document Type
Article
Publication Title
Infection and Immunity
Publisher
American Society for Microbiology
Publication Date
4-1994
Volume
62
Issue
4
Disciplines
Public Health
Abstract
Novel molecular tools and genetic methods were developed to isolate genomic fragments of Mycobacterium tuberculosis that may be associated with virulence. We sought to restore virulence, a characteristic of M. tuberculosis that is correlated with growth rate in mouse spleen and lung tissue, to the avirulent strain H37Ra by complementation. A representative library of the virulent M. tuberculosis strain H37Rv was constructed and transformed into H37Ra. Enrichment for individual faster-growing recombinants was achieved by passage of pools of H37Ra transformants harboring the H37Rv library through mice. A molecular strategy was devised to isolate and clone the H37Rv genomic DNA fragment ivg, which conferred a more rapid in vivo growth rate to H37Ra.
Recommended Citation
Pascopella, Lisa; Collins, Frank M.; Martin, Jean M.; Lee, Mong Hong; Hatfull, Graham F.; Stover, C. Kendall; Bloom, Barry R.; and Jacobs, William R. Jr., "Use of In Vivo Complementation in Mycobacterium tuberculosis to Identify a Genomic Fragment Associated with Virulence" (1994). Public and Community Health Sciences Faculty Publications. 16.
https://scholarworks.umt.edu/pchs_pubs/16