Graduation Year
2017
Graduation Month
May
Document Type
Thesis
Degree Name
Bachelor of Science
School or Department
Biological Sciences, Division of
Major
Biology – Cellular and Molecular Biology
Faculty Mentor Department
Chemistry and Biochemistry, Department of
Faculty Mentor
Brooke Martin
Keywords
YbgL, Chromate, Genotoxicity, Oxidation, Cancer
Subject Categories
Biochemistry | Cancer Biology
Abstract
Cr(V) is a carcinogen that oxidizes guanine aggressively to form spiroiminodihydantion (Sp) and guanidinohydantoin (Gh), both of which contain an unusual hydantoin moiety that cause G→T transversion mutations at a high rate. Endonuclease VIII (nei) can recognize and excise these oxidation products from DNA and is translated as one of five protein products of the Nei operon in Escherichia coli (E. coli). However, the functions of the other four proteins remain unknown. To address this gap in knowledge, we focused on one of the four that immediately precedes nei, the ybgL protein. Previous work by our group has suggested a role for ybgL in vitro. In the current study, we attempt to characterize the role of ybgL by oxidizing a synthetic oligo with Cr(V) and reacting the oxidized oligo with ybgL in the presence of different potential cofactors. Due to the presence of hydantoin moieties within the DNA, we modeled the ybgL protein to the Hydantoinase B class of enzymes, which recognize the hydantoin moiety. This study will attempt to elucidate the role of an uncharacterized protein in excising oxidation lesions caused by chromium toxicity.
Honors College Research Project
1
GLI Capstone Project
no
Recommended Citation
Conen, Mason H.; Martin, Brooke D.; Sugden, Kent; and Whitfield, Savannah, "The E. coli Protein YbgL: A Novel DNA Repair Enzyme?" (2017). Undergraduate Theses, Professional Papers, and Capstone Artifacts. 138.
https://scholarworks.umt.edu/utpp/138
Included in
© Copyright 2017 Mason H. Conen, Brooke D. Martin, Kent Sugden, and Savannah Whitfield