Document Type
Article
Publication Title
Journal of Biological Chemistry
Publication Date
1995
Volume
270
Issue
12
Disciplines
Medical Sciences | Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
Abstract
Arachidonic acid has been proposed to be a messenger molecule released following synaptic activation of glutamate receptors and during ischemia. Here we demonstrate that micromolar levels of arachidonic acid inhibit glutamate uptake mediated by EAAT1, a human excitatory amino acid transporter widely expressed in brain and cerebellum, by reducing the maximal transport rate approximately 30%. In contrast, arachidonic acid increased transport mediated by EAAT2, a subtype abundantly expressed in forebrain and midbrain, by causing the apparent affinity for glutamate to increase more than 2-fold. The results demonstrate that the response of different glutamate transporter subtypes to arachidonic acid could influence synaptic transmission and modulate excitotoxicity via positive or negative feedback according to the transporter(s) present in a particular region.
DOI
10.1074/jbc.270.12.6433
Rights
© 1995 by The American Society for Biochemistry and Molecular Biology, Inc.
Recommended Citation
Zerangue, Noa; Arriza, Jeffrey L.; Amara, Susan G.; and Kavanaugh, Michael, "Differential Modulation of Human Glutamate Transporter Subtypes by Arachidonic Acid" (1995). Biomedical and Pharmaceutical Sciences Faculty Publications. 50.
https://scholarworks.umt.edu/biopharm_pubs/50