Year of Award
2019
Document Type
Dissertation
Degree Type
Doctor of Philosophy (PhD)
Degree Name
Biomedical Sciences
Department or School/College
Department of Biomedical and Pharmaceutical Sciences
Committee Chair
Andrij Holian
Commitee Members
Keith Parker, Howard Beall, Elizabeth Putnam, Horst von Recum
Keywords
Cell fusion, Inflammation, Macrophage, Mouse, Multinucleated giant cell, Particles
Abstract
Multinucleated giant cells (MGC) are homotypic macrophage syncytia associated with granulomas. Despite their correlation with pathology, MGC functional contributions to inflammation are relatively unknown. The objective of this work was to gain an understanding of MGC phenotype. First, techniques were developed to better enable the study of these cells in vitro. Second, inorganic particles known to cause inflammation were observed to cause MGC formation in the lungs. Finally, the particle that resulted in the highest macrophage fusion was used together with the in vitro system to compare MGC and macrophage phenotype in response to stimulation. The results contribute to fundamental MGC cell biology knowledge that is important toward developing approaches to control the foreign body response and understanding the role of MGC in granulomatous disease.
Recommended Citation
Trout, Kevin Lewis, "MULTINUCLEATED GIANT CELL FORMATION AND PHENOTYPE" (2019). Graduate Student Theses, Dissertations, & Professional Papers. 11448.
https://scholarworks.umt.edu/etd/11448
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