Document Type
Article
Publication Title
Modern Research in Inflammation
Publisher
Scientific Research Publishing
Publication Date
8-2015
Volume
4
Disciplines
Medical Sciences | Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
Abstract
We have previously demonstrated that acute treatment with low dose methamphetamine is neuroprotectivein a rat model of severe traumatic brain injury (TBI). Using gene expression analysis, we further showed that methamphetamine treatment significantly reduced the expression of proinflammatory genes after severe TBI. Therefore, to further investigate the potential effects of methamphetamine treatment on the neuroinflammatory response, we examined immunofluorescent staining of Iba1 and CD68, two marker of neuroinflammation, in the rat lateral fluid percussion injury model of severe TBI. In this study, we observed temporal and spatial alterations in the pattern of Iba1 and CD68 labeling within two weeks after severe TBI. In general, methamphetamine treatment did not dramatically alter the pattern of Iba1 and CD68 staining. However, we did observe a unique and significant drug-induced increase of Iba1 labeling within the granule cell layer of the dentate gyrusat 48 hours post injury. We also observed rod-shaped Iba1+ cells within the core lesion in the cortex. These cells showed variable staining with CD68 and aligned most closely with MAP2+ neuronal processes. Thus, acute treatment with low-dose methamphetamine after severe TBI caused a transient bilateral increase of Iba1+ cells within the granule layer of the dentate gyrus but did not alter the overall temporal and regional pattern of Iba1 and CD68 staining within the cortex, periventricular white matter, fimbria, or thalamus.
Keywords
TBI, Methamphetamine, Iba1, CD68
DOI
10.4236/mri.2015.42002
Rights
© 2015 by authors and Scientific Research Publishing Inc.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Smith, D., Brooks, D., Wohlgehagen, E., Rau, T. and Poulsen, D. (2015) Temporal and Spatial Changes in the Pattern of Iba1 and CD68 Staining in the Rat Brain Following Severe Traumatic Brain Injury. Modern Research in Inflammation, 4, 9-23. http://dx.doi.org/10.4236/mri.2015.42002